diff --git a/population.py b/population.py
index 0769839fc70466164fe07d202d0cc72997c222e5..1a9301aef80ada02598b9a556590f604a64e93c4 100644
--- a/population.py
+++ b/population.py
@@ -163,8 +163,6 @@ class Population:
             y=copy.deepcopy(newself[t])
             couple_enfant = enfant(x,y)
             for child in couple_enfant :
-                lineList = [line.rstrip('\n') for line in open("plasmid_8k.fasta")]
-                brin = ''.join(lineList[1:])
                 child.mutation_close_values(proba_mutation, number_of_mutations = 2)
                 child.evaluate()
             newself.append(couple_enfant[0])