diff --git a/population.py b/population.py
index ac2fba17b0c4d08ed24302b06e3ccbdff4a0c9d5..618126d9c9589a39ab2ccd3748595efb0fc63e43 100644
--- a/population.py
+++ b/population.py
@@ -136,8 +136,6 @@ class Population:
             y=copy.deepcopy(newself[t])
             couple_enfant = enfant(x,y)
             for child in couple_enfant :
-                lineList = [line.rstrip('\n') for line in open("plasmid_8k.fasta")]
-                brin = ''.join(lineList[1:])
                 child.mutation_close_values(proba_mutation, number_of_mutations = 2)
                 child.evaluate()
             newself.append(couple_enfant[0])